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Mean NIR fluorescent intensity of the double-positive cell population was normalized to mean green fluorescence intensity of the co-expressed EGFP to account for transfection efficiency.
The collected data was analyzed using FlowJo software version 7.2.5 to determine the mean green fluorescence intensity after each treatment.
Mean green fluorescence of live cells incubated with each antioxidant showed a significant uptake of Proxison and quercetin (n = 2).
Mean green fluorescence was calculated with FlowJo X. SH-SY5Y cells were seeded at 6.25 × 104 cells/cm2 on 13 mm coverslips.
The collected data was analyzed using FlowJo software version 7.2.5 for Microsoft (TreeStar, San Carlos, CA, USA) to determine the mean green fluorescence intensity after each treatment.
The results showed that the mean green fluorescence intensity and M RNA transcripts were significantly reduced, and that the expression of M glycoprotein was strongly inhibited in those cells co-transfected with M-specific siRNAs.
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BL, bright light; Red, chlorophyll auto-fluorescence; Blue, mBFP blue fluorescence; Green, green fluorescence of MitoTracker.
and presented as the geometric mean of green fluorescence for carboxy-H2DCFDA or red signal for MitoSOX™ red.
Mean intensity of green fluorescence in cells in three microscope fields per well and three wells for each condition was used to determine average Htt levels.
The mean ± SD green fluorescence intensity per cell was obtained from approximately 200 cells per sample using Image-Pro Plus, version 6.0 (Media Cybernetics Inc., Bethesda, MD).
To generate the final HIV-1 shRNA-library, the shRNA-sequences were subcloned by LR recombination into the lentiviral vector pLP/EGFP/shLib (Fig. 1e, final library) which allows for the enrichment of transduced cell lines by puromycin selection and fluorescence monitoring by means of enhanced green fluorescence protein (EGFP).
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