The mean 1000 grain weight was similar for Agrobacterium-silenced and control lines, as was the mean weight of T1 roots, although in this case only for plants from experiment 4. Additionally, CKX activity in bulked samples of T1 roots, obtained after Agrobacterium-mediated silencing, in contrast to the data from the biolistic method, was decreased by over 29% compared to the non-silenced control.
Similar lower data were obtained for 1000 grain weight and mean weight of T1 roots in transgenic plants of Golden Promise compared to the control.
For 100 grain weight (GW), the mean of the population in both conditions was unchanged (Tables 1 and 2).
Statistical analyses were based on phenotypic data of average grain size and grain weight in two environments.
The maximum and mean contents of Z + ZR, IAA, and ABA were positively very significantly correlated with the maximum and mean grain-filling rate, filling degree, and grain weight.
In the P-GSTA method, a linear function with all significant granulometric parameters that are summed with different weighting factors was used to infer sediment-transport direction (sediment flux pattern); the previous grain-size trend analysis (GSTA) methods considered only three parameters (mean grain size, sorting, and skewness) with equal weighting.
Mean grain yield in this study (10.1 Mg ha−1 dry weight) was representative of the average yield (10.0 Mg ha−1) for the area (Story County, IA) and year (2005).
Student t-tests (assuming unequal variances) were used to determine whether the means of the samples in the segregating T1 populations for seed set and 1000 grain weight were statistically different (Microsoft Office Excel 2003).
The mean grain sizes of applied solids were obtained using photon correlation spectroscopy (Zetasizer 3000, Malvern Instruments).> Polyacrylic acid (Fluka) with the weight average molecular weights 2,000, 100,000, and 240,000 was used in the experiments.
