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Mean fluorescent density (in arbitrary units) of each band was measured by the manufacturer-supplied software.
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Monocyte human leucocyte antigen-DR (mHLA-DR) membrane density (geometric mean fluorescent intensity) was characterised using flow cytometry following a 20-hour incubation.
Finally, we evaluated Pv parasite density and mean fluorescent signal intensity (a measure that correlates with the intensity of Pv infection) over time in an analysis of repeated measures using generalized estimating equations [29].
Both background fluorescence and mean fluorescent intensities of ROIs were calculated in each image.
Mean fluorescent intensity (MFI) was calculated as a ratio of mean fluorescence sample/isotype fluorescence.
The density of the C5aR, as determined by the mean fluorescent intensity on monocytes already expressing the receptor, was unchanged (data not shown).
The mean fluorescent intensity (MFI) was measured with flow cytometry.
(F) Bar graph quantification of the mean fluorescent intensity analyzed using the FlowJo software v7.6.1.
(D) Bar graph quantification of mean fluorescent intensity analyzed using the FlowJo software v7.6.1.
Fig. 4 Correlation between the mean fluorescent intensity (MFI) of PMAP-1 and polymorphonuclear neutrophil (PMN) activation (CD11b expression).
Telomere length was expressed as mean fluorescent signal intensity.
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