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The geometric mean fluorescence modulation ratio is calculated as: (MFI with modulator - cold control MFI /(MFI with diluent control - cold control MFI) [ 53, 54].
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Mean fluorescent intensity (MFI) was calculated as a ratio of mean fluorescence sample/isotype fluorescence.
Fluorescence values of individual cell-mimics were mean fluorescence values of the individual segmented cell-mimics.
Assay values were reported as mean fluorescence intensity (MFI).
The median DSA mean fluorescence intensity (MFI) was 3197.
The mean fluorescence from each image was calculated with ImageJ.
(A,B) Colonization is quantified as mean fluorescence intensity (MFI).
Fluorescence was measured on a BD FACS Canto II flow cytometer, and results are shown as geometric mean fluorescence expressed in arbitrary fluorescence units (AFU).
Higher coverage (311) surface regions showed no significant fluorescence modulation nor any difference based on state of hybridization.
Mean fluorescence of an ROI spanning a single neuron measured over 16 heat pulses.
Quantification of the mean fluorescence intensity (MFI) is shown in the bar graph.
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