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The mean fluorescence lifetime of this mutant also increased by 70% on the addition of glucose.
Based on discernable diminution of HSA mean fluorescence lifetime as a function of biluminophore concentration, facile occurrence of fluorescence resonance energy transfer (FRET) is substantiated as the probable quenching mechanism accompanied by structural deformations in the protein ensemble.
However, since 48 h after the microcapsule fabrication, further changes in the mean fluorescence lifetime were insignificant.
Small differences between the mean fluorescence lifetime of tumor and contralateral sites were observed in tumors with low-level (+1) expression of HER2 receptor.
Small change between the mean fluorescence lifetime of the tumor and contralateral sites was observed in tumors with low-level of HER2 receptors perhaps due to the small ratio of bound to unbound receptors inside the tumor.
Results are expressed as mean fluorescence lifetime ± SEM.
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In conclusion, degeneration of the RPE with subsequent photoreceptor degeneration by NaIO3 lead to longer mean fluorescence lifetimes of the retina compared to control mice, whereas during specific degeneration of the photoreceptor layer induced by MNU shorter lifetimes were measured.
The mean fluorescence lifetimes of Trp68-only and Trp156-only are always very short (∼0.1 ns), indicating that these residues are highly quenched.
If a molecule samples multiple states, the FRET intensity indicator (FD/FA) and the average fluorescence lifetime (〈τD(A)〉f) would differentially reflect changes to the mean and variance of the photon counts, respectively.
This involved the fluorescence lifetime of the individual FRET standard-expressing cell and the average fluorescence lifetime of a donor-only control cell subset with comparable fluorescence intensity.
The average fluorescence lifetimes of uninfected cohorts was 270±30 ps (mean±SD; N = 45).
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