Cells displaying Notch molecules were expressed as either percentage of positive cells, in case of bi-modal fluorescence distribution, or mean fluorescence intensity (MFI), after electronic gating of CLL cells, hBM-MSCs or hBM-MSCs*.
Correspondingly, the fluorescence threshold for the detection of an infected cell was set equal to the quantile (mean x0.99) of the cumulative fluorescence distribution (F) of mock infected cells.
Dye uptake was expressed as a D value ranging from 0 (no difference) to 1 (no overlap) generated by a Kolmogorov-Smirnov test, which was used to determine the distribution of the mean fluorescence intensity (MFI) between the presence and absence of each modulator.
This data was then used to define the 99.7% confidence intervals for the distribution of fluorescence ratios corresponding to each genotype based on three standard distributions around the mean fluorescence ratio for each genotype (see boxes in Figure 6).
In the first experiment, control replicates sets for each genotype were used to define the 99.7% confidence intervals for the distribution of fluorescence ratios corresponding to each genotype based on three standard distributions around the mean fluorescence ratio unique to each genotype.
The distribution of ratio of mean fluorescence intensities (log2 R) of hemizygous genes (i.e., genes within the deletions) is significantly different from that of the genes outside of the deletions (P < 10−12, fig. 2 A ).
However, influence of the measuring approach onto the deduced mean cell fluorescence or onto the reconstituted cell fluorescence distribution in the analyzed population is often not considered.
Right panel, the distribution of nuclear Ki-67 mean fluorescence in the SOX2− and SOX2+ cells in DMSO-treated (green) and erlotinib treated (red) cells is displayed.
In case of uni-modal fluorescence distribution, MFI was calculated as the geometric mean of fluorescent intensity of hBM-MSCs, hBM-MSCs* or CLL cells expressing Notch molecules.
Decitabine alone or in combination caused H2DCFDA fluorescence distribution peak broadening but only moderate mean fluorescence value shift in CML-T1 cells.
