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It is well known that mean fat cell volume and weight differs between various adipose regions in man.
The mean fat cell volume and size were calculated by formulas developed by Hirsch and Gallian [29].
Using direct microscopy, the diameter of 100 cells was determined and the mean fat cell volume was calculated.
Mean adipocyte number was determined by dividing the mean fat mass by the mean fat cell size as described previously [14].
Total number of fat cells in the body was calculated as the amount of body fat divided by the mean fat cell weight.
Mean fat cell size and volume were determined on isolated fat cells as follows: Fat cell diameter was measured in direct microscopy and averaging the diameter of 100 cells in each individual or mouse.
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The unadjusted mean average fat cell volume in each individual was also investigated and related to the amount of body fat.
The protein, called transforming growth factor-beta 2, or TGFB2, is an adipocytokine (which literally means "fat cell movement") that seems to lower blood sugars in mice.
The effect of α-tocopherol on corticosteroid-induced adipogenesis was assessed by comparing the mean bone marrow fat cell size and the mean bone marrow fat cell area in the control group and in the experimental group.
The mean values are essentially the same as those determined from fat cell sizing of intact pieces of human adipose tissue [30].
Each fat cell harbors a typical capacity of only about 500 micro-calories, which means even a lean body still needs about 4 million fat cells to store the equivalent of a Tic Tac.
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