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The mean extraction efficiency for S and R--bufuralol from plasma was in the range 97 102% at 15 400 ng/ml level for each enantiomer.
Mean extraction efficiency was 73%% and final concentrations were corrected for individual extraction efficiencies.
Calibration curves were linear within the concentration ranges relevant to this study, with precision and accuracy within 15% and mean extraction efficiency of 99.7±12.7%.
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The mean extraction efficiencies of tilianin and acacetin obtained under the optimized conditions showed good repeatability with calculated RSD values of 4.02 and 3.90%, respectively.
The absolute matrix effect and extraction recovery were calculated as follows: Absolute matrix effect = (Mean peak area)set 2 ⁄ (Mean peak area)set 1. Extraction efficiency = (Mean peak area)set 3 ⁄ (Mean peak area)set 2. This post-extraction spike method proposed by Matuszewski et al. [11] provides a quantitative understanding of the level of matrix effect observed for specific analytes.
Steroid extraction efficiency (mean percentage ± standard deviation = SD of recoveries) was included (Methods subsection "Reproductive stages"): Testo: 70.9 ± 2.7, DHT: 73.7 ±4.3, E2: 59.9 ±4.0, P4: 55.9 ±11.0.
DOX recovery (extraction efficiency), measured through mean area from six replicates of extracted samples vs. extracted drug free plasma samples (both spiked before extraction), was determined at each individual levels (LQC, MQC and HQC).
In this section, we discuss two mechanisms that have been suggested to explain the resonant-coupling enhancing effect, namely, increase of IQE via emitter/plasmon coupling and increase of light extraction efficiency [LEE] by means of out-coupling of the generated photons.
Then, we took the average value on the extraction efficiency.
The main factors affecting the extraction efficiency have been optimised by means of a central composite design.
The first 10 samples were extracted three times to determine the extraction efficiency.
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