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Furthermore, the expression level was normalized by Z-transformation (the mean expression was set to 0 and standard deviation to 1 for each gene in each dataset).
First, the mean expression level for each miRNA in all experiments was calculated, and then a 2nd degree polynomial function F for fitting each experiment signals to the calculated mean expression was found.
For galectin-3, mean expression was -0.3 (SD 0.88) and -0.9 (SD 0.53) respectively.
If two probes met these criteria for one gene, the probe with the highest mean expression was chosen.
For visualization the mean expression was determined across the heterozygous samples, which was then subtracted from the expression levels for the individual samples.
For almost all mutants, the mean expression was within 50% of WT and any reduction was not statistically significant (Table 2012).
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Evolution of CUP1 toward higher noise in its expression is conceivable: High mean expression is both advantageous in high copper concentrations and disadvantageous for fermentation.
When significant differences in mean expression were identified in patients with disease and without disease, the prognostic significance was determined by log-rank tests (Kaplan Meier curves).
Our results showed that the negative correlation between transcriptional noise and mean expression is not determined by distal CRMs but is solely promoter derived for vertebrate Msx1.
Also, the mean expressions was significantly higher in node 2 (P = 0.02) than in the normal tissues.
After normalization, mean expressions were shown as fold increase (± SEM), after comparison with the mean expression levels of the WT control to which was ascribed an arbitrary value of 1.
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