For each gene, we calculated its mean expression level and the standard deviation across the 45 tissues covered in UniGene.
This was biased toward the sense strand, correlated with mean expression level, and clustered near breakpoints.
At least three replicates were performed, and the mean expression level for each gene was calculated.
The mean expression level of tumor necrosis factor-α and interleukin-6 mRNA in the JTE-607 group was lower than that in the HCl and NS groups.
Controlling the mean expression level of a gene while reducing its noise is a challenge in many applications of Synthetic Biology.
In previous works, we proposed a gene synthetic circuit to reduce gene expression noise while achieving a desired mean expression level.
We also calculated the mean expression level of each gene.
But this effect is confined to a narrow range, essentially a factor of two in mean expression level.
The mean expression level of BART16-3p wapproximatelyely 20-fold higher than that of BART16-5p, similar to the read count ratio detected in T10 (Table 2).
Figure 1 shows the mean expression level differences of known genes, which gave a signal in at least two experiments in one group.
For this purpose, we calculated the log ratio between human-orangutan and chimpanzee-orangutan squared mean expression level differences for each probe set.
