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Despite the accuracy of the classifier for random forest-based class assignment in a test set of DC samples, the mean expression index of transcripts was low.
The mean expression index for each duplicate was used for statistical analysis.
Interestingly, also the decay rates of the density distributions and the mean expression index <φ> of about 2.1 are very similar for all considered cases.
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Coexpression index and mean co-expression index values were calculated from 10,000 randomized genome data (see Methods).
The P values for both mean co-expression index values were below 0.00001 (Supplementary Fig. S2 [see Additional file 2] and S3 [see Additional file 3]).
The derived Monte-Carlo distributions (Supplementary Fig. S2 [see Additional file 2] and S3 [see Additional file 3]) allowed determination of the P value for the coexpression index of a given gene or the mean co-expression index of a given set of genes.
The abbreviation used in this work, a brief gene description, the mean of reads per kilobase of transcript (RPK) and the mean of the expression indices (EI, N° of transcript per FD cell) are reported.
The PAM50-PROLIF index is the mean expression of 11 proliferation-relatedelated genes of the PAM50 assay [ 23].
If we calculate the mean ribosomal expression ratio as a global RP expression index, all tissues showed similar values (0.74 0.99) except brain with only 0.48.
4) Calculate mean expression level over all genes studied for each sample type as an index of both experimental and biological conditions that promote high levels of measured expression.
The expression values were log 2 transformed after calculating the expression index.
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