First, the mean expression level for each miRNA in all experiments was calculated, and then a 2nd degree polynomial function F for fitting each experiment signals to the calculated mean expression was found.
The other 10 genes were differentially expressed, but displayed various degrees of up- and down-regulation in comparison to the mean expression level.
We generated simulated case-control expression data that spanned a range of sample sizes, differential expression magnitude (the difference between case and control sample distribution means), and differential expression degree.
The so-called degradation hook-plot, shown in Figure 1a and b, displays this 3′/5′ intensity difference in dependence on the mean logged probe intensity approximating the expression degree of the respective gene.
fMean square of deviation of means from estimated regression line (MS-reg), which estimates the degree to which genes deviate from the linear model in their level of mean expression for a particular tissue sample.
By plotting the mean expression values for hTERT and cMyc in a series of separate WT1 transfections, we found a strong correlation, indicating a similar degree of inhibition.
The differences in gene expression profile between the three explosive treatments largely come from the degree of alteration of the 329 significant genes as evidenced by the high correlation in both absolute and relative mean expression (Table 3).
The CV is used as a statistic for comparing the degree of variation between genes, even if the mean expressions are drastically different from each other.[16] The calculated CVs for all genes were ranked.
Due to significant variance in the values of forward degrees, we derive expressions for an expected or mean forward degree of a node relative to its distance in terms of hops from the source.
I mean, to what degree?" "To no degree," Deb says.
