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Mean elasticity of the cell membrane was then quantified for each sample collected.
Invasive cancers with an ultrasound size <15 mm had an average mean elasticity of 109 kPa, compared with an average value for lesions ≥ 15 mm of 167 kPa.
The mean elasticity of malignant lesions was significantly higher than the mean value of benign lesions (170 ± 41.6 kPa versus 62 ± 21 kPa).
A cutoff value for mean elasticity of 50 kilopascals (kPa) was used for benign/malignant differentiation on shear wave elastography, as this level had been validated in a previous study (Evans et al, 2010).
While control cells were found to have a mean elasticity of 1.6 kPa, nocodazole-treated cells were nearly 2-fold stiffer at a mean stiffness of 3 kPa, consistent with the observation that cells develop stress fibers under these conditions.
The performance of shear wave elastography combined with BI-RADS classification, whereby a lesion with either BI-RADS 4 5 greyscale scores or an averaged mean elasticity of over 50 kPa was classed as malignant, was also assessed.
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The mean elasticities of Q to P, E0 and m were 2.66, − 1.66 and − 3.17, respectively.
In contrast, we found the results for mean elasticity to be superior than those found using maximum elasticity.
ICCs for maximum lesion elasticity, mean elasticity and ratio of lesion-to-fat elasticity were also nearly perfect (ICC = 0.88; 0.91; and 0.83 respectively).
Significance of difference between mean elasticity value between groups of nodules was calculated by t-test for independent samples.
Measurement of mean elasticity yielded an intraclass correlation coefficient of 0.99 for two observers assessing the same pairs of elastography images.
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