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For each subject, echo planar images were aligned to each other; the T1 co-registered to the mean echo planar image and normalization parameters (obtained from T1 segmentation) applied to all echo planar images (3 × 3 × 3 mm resolution), followed by smoothing (9 mm Gaussian kernel).
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T1-weighted structural images were co-registered with the mean echo-planar images and normalized to a standard T1 template.
Images were realigned and the resulting mean EPI image was used to estimate normalization parameters to the standard Montreal Neurological Institute echo planar image (MNI EPI) template, which were then applied to all EPI volumes.
The mean image generated was then spatially normalized into standard stereotactic space, using the Montreal Neurological Institute MNII) echo planar imaging (EPI) template.
Anatomical images were acquired by means of 3D-MPRAGE sequences and f-MRI images were obtained by means of echo-planar imaging.
Echo planar imaging.
fMRI data are usually acquired using echo planar imaging (EPI).
The method is analogous to echo planar CSI but utilizes conventional gradient echoes, exploiting the principle of spectroscopic bandwidth extension by interleaving temporally offset gradient-echo trains.
BOLD fMRI data were collected using gradient-echo echo planar imaging with 160 volumes at repetition time (TR)¼ 3 s.
Echo-planar images were collected using a single shot, gradient-echo, echo planar pulse sequence (field of view (FOV) = 24 cm, echo time (TE) = 35 ms, flip angle (FA) = 90°, in plane resolution = 64×64).
Diffusion MRI data were collected with a diffusion-weighted, multi-shot (four segments), spin-echo echo planar imaging (EPI) sequence.
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