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Due to the uneven distribution of points across illumination, the average mean depth for 10% illumination intervals were calculated for the regression analysis.
The mean depth for the filtered SNPs was 3.3.
The average mean depth for the targeted regions was 246.2 ± 14.9; 98.7 ± 0.1% of the covered exons had ≥10 reads.
A total of 708.3 million read pairs corresponding to 77.9 Gb DNA were obtained (13.7× mean depth for each line).
The mean depth for each accession was about 0.50 × on average (ranging from 0.27 × to 0.96 ×) (see Additional file 8).
The average mean depth for the targeted regions was 311.8 ± 86.3; 98.4 ± 2.9% of the exons had a coverage ≥10 reads.
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To demonstrate the expected higher coverage of MiSeq with WGBS, we calculated the mean depth of coverage for each CpG locus, within each amplicon, for each group (Fig. 5).
To describe potential GC bias in sequencing runs, we calculate the mean depth of coverage for each GC content bin (0 100 representing 0 100% GC composition) for a series of windows along the genome (or, in the case of targeted sequencing experiments, within targeted regions).
Mean depth of leakage for the groups with the smear layer intact was 2.5 ± 1.0 mm for passive dye penetration, 6.7 ± 2.8 mm for vacuum dye penetration, and 3.0 ± 1.1 mm for fluid filtration dye penetration.
Before their removal, the mean depth of coverage for targeted regions was 383 reads, but after duplicate removal the mean depth amounted to 110 reads with base phred quality scores of at least 17.
The mean depth of coverage for sequence reads to the EAV-HP LTR was 12.58 ± 1.08 in Ethiopian chickens, 7.38 ± 2.33 in Pirbright lines, and 16 ± 2.83 in the SRA data, respectively.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com