Sentence examples for mean cycle threshold from inspiring English sources

Mean cycle threshold values (Ct values) for each target gene were normalized with mean beta-actin Ct values.

Mean cycle threshold (Ct) values for all miRNAs were quantified with sequence detection system software (SDS Version 1.02; Applied Biosystems).

(7 of 13, 54%) and s.c. (5 of 11, 45%) group, as were the mean Cycle threshold (Ct) values (35·86 cycles and 37·52 cycles, respectively).

A standard curve was generated from the mean cycle threshold (Ct) of eight standards (1∶5 serial dilution) of a known concentration in triplicate, while amplification and dissociation curves were generated for all standards and samples (Applied Biosystems, California, USA).

Threshold cycle number was determined using Opticon software and ER levels were normalized to β-actin levels using the formula 2− (Et−Rt) where Rt is the mean cycle threshold for the control gene and Et is the mean threshold for the experimental gene.

To confirm whether CNAs levels reflect disease morbidity, the sum of scores attributed to each patient (Table S1) was plotted against the CNAs levels detected in plasma with the Quant-iT™ Pico Green or the mean cycle threshold detected by qPCR amplification of the hTERT genomic sequence (Fig. 2B).

The transcript concentrations were calculated relative to the endogenous control β-actin (Actb) as 2 − (C t G e n e − C t A c t b ), where Ct Gene and Ct Actb correspond to the mean cycle thresholds for the test gene and β-actin, respectively [ 39].

Out of all miRNAs investigated, 153 miRNAs could be unambiguously detected; a mean cycle of threshold (CT) value of 35 cycles was defined as a cut-off for reliable miRNA detection (CT value defines the threshold, i.e. the cycle number when exponential PCR product amplification occurs; Table S1).

Incubation of RT with aptamer and RT-step at 31 °C resulted in a mean delayed cycle threshold of up to ∆C t 7.31 (50 μM aptamer, Fig. 1a) compared to the reference without aptamer, indicating reduced RT activity (Fig. 1a, b).

The mean minimal cycle threshold values (Ct) were calculated from quadruplicate reactions.

The results of RT-PCR from triplicate experiments were expressed as mean Ct (Cycle threshold) values and standard deviation.