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Figure 4 shows histograms of the mean current amplitude of translocation events measured for aggregated ABTS+ at different voltages.
Figure 4a d shows histograms of the mean current amplitude of translocation events measured for PS microspheres at voltages applied (from −600 to −900 mV).
Fig. 4 The histograms of the mean current amplitude of translocation events measured for aggregated ABTS+ at different voltages (−400, −500, −600, −700, −800 mV) in 0.1 M KCl, 0.1 M PBS, pH 7.0.
Current-voltage (I/V) curves were constructed by plotting the mean current amplitude for each clamped potential.
Leak current was subtracted by measuring the mean current amplitude in the last 2 ms of the hyperpolarizing voltage step and subtracting a correspondingly scaled Ohmic current from each recorded current trace.
For mIPSCs, representative traces (A ), mean current amplitude (B ) and mean frequency (C ) are shown.
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Fig 4C summarizes the mean current amplitudes recorded at −60 mV under different experimental conditions.
(E, F ) Distribution of mean current amplitudes (E ) and paired-pulse ratios (F, PPR) for 38 connected pairs.
I/V relationships were expressed as mean current amplitudes measured at all potentials at 50 or 350 ms after the pulse onset.
(D ) Current-voltage relation of recordings from panel C. (E ) Mean current amplitudes at pHi 6.2 and pHi 7.3 before and after application of NH4Cl (10 mM) (n = 3).
Comparing the mean peak current amplitude across early postnatal weeks demonstrates the developmental upregulation of nicotinic excitation in male and female layer VI neurons as illustrated in Figure 1B.
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