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Data are expressed as stimulation index (SI), calculated by dividing the mean cpm obtained in the stimulated cultures by the mean cpm obtained in the control cultures.
An SI ≥3.0 and a Δcpm >1,000 (mean cpm obtained in cultures with antigen minus mean cpm obtained in cultures incubated in the absence of antigen) was regarded as a positive response.
Results were expressed as stimulation index (SI) defined as the ratio of mean counts per minute (cpm) obtained in cultures with antigen to mean cpm obtained in cultures incubated in the absence of antigen.
Similar(57)
Results were expressed as counts per minute (cpm), and positivity of each sample was calculated by subtraction of the mean cpm value plus three times the SD obtained for the negative control.
The immunostimulatory activity was compared between PDAC patients and healthy controls by dividing the counts per minute (CPM) of patients with the mean CPM of controls and the CPM of the controls with the mean CPM of the patients for each plate analyzed.
Reported DEG were obtained by requiring q-value <0.05, abs[log2 fc) > 1] and mean CPM > 20 in at least 1 cell type/condition.
Spontaneous proliferation of PBMCs was considered when a mean CPM value ≥ 500 due in triplicates (i.e., three times the mean counts per minute measured in uninfected control cells) was obtained.
The proliferative response is expressed as a stimulation index (SI) calculated by dividing the mean cpm of peptide-stimulated wells by the mean cpm of non-stimulated wells.
The proliferative response is expressed as a stimulation index (SI) which is calculated by dividing the mean counts per minute (cpm) of peptide-stimulated wells by the mean cpm of non-stimulated wells.
The results were expressed as Δcpm (DCPM) (mean cpm stimulated cultures – mean cpm unstimulated cultures) [19].
Results were expressed as the mean cpm of triplicate wells.
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