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The mean coverage was obtained by dividing the 286,454,112 bp of sequence that maps to the assembled genome by the 258,954,041 mappable bases of the assembled genome.
After sequencing, mean coverage was >95× in all samples.
The resulting mean coverage was 221x (patient 1), 168x (patient 2) and 194x (patient 3).
The mean coverage was 92× for tumor tissue and 65× for normal tissue.
The mean coverage was 156.7 with minimum 67 and maximum 904.
Consequently, the median coverage rather than the mean coverage was used.
Similar(40)
This fragmentation is a genuine feature of the genome, supported by an assembly in which the mean coverage is about 400X.
In other words, 56% of the variance in mean coverage is accounted for by the value of ANC 4+.
Consistency between different capture technologies suggests that the reduced coverage reflects the local sequence context, making these regions vulnerable to sequencing error when the mean coverage is low.
The fraction of bait-covered exons in the genome achieving coverages equal or lower than the overall mean coverage is indicated on the y-axis.
Proportion of mean coverage is a measure relative to the depth of coverage of the reference reads mapped backed to the reference assembly.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com