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Mean coverage of the targeted exons was 697X.
Fig. 2 Ordination by detrended correspondence analysis (DCA) based on the mean coverage of all species.
Approximately 78%% of them corresponded to unique reads (Table 1) while the mean coverage of these was x36.
By sequencing, a mean coverage of 82 % was reached, 10× coverage was 97.5 % for target sequences and 30× coverage was 87.1 % on target (Additional file 1: Table S1).
The WES generated 34,600,000 bp of nucleotide sequences mapping once to the targeted exome, which achieved a mean coverage of 122×of the target exome.
The mean coverage of the exomes was 74 times.
Mean coverage of BAC pools reached nearly 35x.
Then, the difference in gene expression of a contig between cultivated and wild carrots was calculated as (mean coverage of cultivated carrots - mean coverage of wild carrots)/ mean coverage of cultivated and wild carrots).
The final TopHat-Fusion-specific fusion has a simulated mean coverage of only 2.86.
In total, the mean coverage of mapped reads across both strands was 32 x.
Overall, there was high mean coverage of individual alleles for all loci.
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