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This fragmentation is a genuine feature of the genome, supported by an assembly in which the mean coverage is about 400X.
In other words, 56% of the variance in mean coverage is accounted for by the value of ANC 4+.
Consistency between different capture technologies suggests that the reduced coverage reflects the local sequence context, making these regions vulnerable to sequencing error when the mean coverage is low.
The fraction of bait-covered exons in the genome achieving coverages equal or lower than the overall mean coverage is indicated on the y-axis.
Proportion of mean coverage is a measure relative to the depth of coverage of the reference reads mapped backed to the reference assembly.
These sets are obtained when mean coverage is equal to 100 and comprise the best reconstruction achievable using these methods with standard parameter settings.
Similar(52)
We obtained 15.4 mln paired-end Illumina reads, on average 77.3% (SD 4.7%) reads were on target and the mean coverage was 2080×.
The mean coverage was 142-fold (median of 142-fold) and 84.1% of the target sequence was covered at a minimum of 10 ×.
The mean coverage was obtained by dividing the 286,454,112 bp of sequence that maps to the assembled genome by the 258,954,041 mappable bases of the assembled genome.
After sequencing, mean coverage was >95× in all samples.
Consequently, the median coverage rather than the mean coverage was used.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com