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The mean coverage for the leptin gene was ~ 80× [26].
The mean coverage for transcribed genome sequences and gene models was 21 and 27, respectively.
The latter were prepared in order to obtain higher mean coverage for these samples.
The mean coverage for bases within target regions was 70-fold and 92 % had at least 10-fold coverage.
Table 1 shows the minimum, maximum and mean coverage for the individual genotyping of the three nuclear loci.
The mean coverage for each transcript was then calculated by averaging the coverage for each nucleotide within the transcript.
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Although SHS used shorter, unpaired sequence reads, more MPG indels were determined than in FS (due to higher total mean base coverage for SHS).
If we assume the need for 100x total mean base coverage for sensitive genotype determination, this would require at least 155 million 100 base pair reads for chromosome X.
The average mean coverages for the sequenced strains 1121/01, 2451/01 and 1307/01 were 293, 224 and 234, respectively.
The alignment resulted in a mean coverage of 14.02X for the Duelmener, 12.21X for the Sorraia, 13.37X for the Arabian, 10.97X for Hanoverian 2 and a higher mean coverage of 25.38X for Hanoverian 1 due to its run on two lanes (Additional file 1B).
In fact, it means coverage for what an employer has chosen to pay.
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