Exact(11)
Black lines: mean coverage at common peaks.
C. Mean coverage at peaks for each experiment.
Dash line: mean coverage at detected peaks that were not in common with other experiments.
Grey line: mean coverage at undetected peaks that were called only in other experiments.
Mean coverage at common peaks was almost always higher than at sample specific peaks, confirming that intersecting peak lists indeed selects peaks of higher signal to noise ratio.
In a handful of exceptional cases, mean coverage at undetected peaks was very close to mean coverage at sample specific peaks (experiment 1 for Rad21 ChIP-seq in HepG2, Fig. S53C), or peaks were called in one experiment despite a low read enrichment (experiment 1 for NRSF ChIP-seq in HepG2, Fig. S36C).
Similar(49)
Based on the simulation data, a mean coverage of at least 30X is needed to maintain a reasonable FDR for Scalpel.
99.6% of the targets had a mean coverage of at least 1X, 89.3% had a mean coverage greater than 5X, and 40.3% had a mean coverage greater than 10X.
Using the eight SSC samples, we investigated the coverage behaviors of the WGS and WES data by the following: distribution of the read depth, mean coverage, coverage fraction at X reads, coefficient of variation (C v ) (See Methods).
After Illumina MiSeq pair-end sequencing, 4.1 to 5.2 million reads were generated from each sample with a mean coverage depth of at least 8,300x (Table 1).> Reads from the same amplicon with the same molecular barcode were processed into one consensus read.
Genomic DNA was prepared from a single cell clone of this stock and sequenced on an Illumina HiSeq2000 instrument; 6.35×10 100 base pair reads were obtained with paired-end sequencing, of which 92.8% could be aligned to the Galgal4.73 reference genome, and 5.950×10 base pairs were mapped over the 1.072×10 base pair reference genome at 55× overall mean coverage, with a peak at 52×.
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