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During the procedure, heparin was continuously administered and adjusted to prolong the partial thromboplastin time (PTT) 1.5 2.0 times the mean control value.
However, in one MBP-exposed animal, the ratio was well outside the control range (∼3× the mean control value).
The mean control value (corresponding to non electrotransferred muscle) was multiplied by 2 and subtracted from each sample value.
There was no significant change in MBP-exposed animals, although in one animal, the percentage of OCT4+ cells was far above the control range (5× the mean control value) (Fig. 3B).
The deviation, and the mean control value map on to the actual discrete step value of control at that stage.
Each element of a deviation vector corresponds to a control stage, and is a deviation from some "mean" control value randomized initially for that stage.
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The genetic operations are periodically followed by the replacement of mean control values by a newly available optimal control solution, and by the size-variation of control domain between its limits.
The mean control values +2SD were regarded as cut-off between normal and pathologically elevated cytokine levels (cut-off values: TNF-α: 2.8 pg/ml; IL-1β: 1.63 pg/ml, IL-6: 12.5 pg/ml; TGF-β1: 150 pg/ml).
This equaled to 26.4% (p < 0.01) and 34.4% (p < 0.01) reduction of CPS levels, respectively, as compared with the mean control values.
Viability results were expressed as a percentage of mean control values resulting in the calculation of the 50%% growth inhibition (GI50).
In the AC, neuronal loss was only significant in bvFTD cases compared with both controls (means ± standard deviation total number of 55,555,584 ± 21,086,167, bvFTD 39 ± 10% of mean control values, p < 0.05) and AD (74 ± 11% of mean control values, p = 0.05), with the loss observed only in cases with FTLD-tau (45% more neuronal loss in AC than those with FTLD-TDP, p < 0.05, Figure 1A).
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