Data normalized to the activity of firefly luciferase that is co-expressed from the dual reporter and to a negative control miRNA vector and presented as mean + SEM.
To quantify the impact of SNX5 depletion on macropinocytosis, the mean number of TxR-dextran labelled macropinosomes per cell was determined for BMM expressing control miRNA and miRNA-1.4 with low levels of SNX5.
Furthermore, the WIF1 level was upregulated after overexpression of miRNA-137 compared with miRNA-137-NC (control miRNA) in GH3 cells.
Luciferase activity was measured in the presence of either the test miRNA or a control miRNA.
For each miRNA the signal intensity was normalized to a negative control miRNA (z-score).
ncRNA, negative control miRNA.
Correlation of control miRNA profiles across beadchips.
Disease control miRNA expression resembled that in healthy control individuals.
A nonspecific control miRNA (Pre-miR miRNA Negative Control #2; Ambion Inc).
For pDC315-GFP-miRNA constructs, control miRNA, miRNA-1.4 and miRNA-2.4 were amplified from pcDNATM 6.2GW/EmGFP control miRNA, pcDNATM 6.2GW/EmGFP-miRNA-1.4 and pcDNATM 6.2GW/EmGFP-miRNA-2.4, respectively.
