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De novo assembly generated a total of 2,326 contigs (contig range size = 100 8,697 bp; mean contig size = 457 bp; N50 = 825 bp) and was submitted to the DDBJ/EMBL/GenBank Whole Genome Shotgun project when the contig size was longer than 500 nucleotides under accession number JWHF00000000.
The mean contig size was 607 bp.
The mean contig size was 634 bp, and the contig size ranged from 201 to 28,343 bp.
After the first assembly, 411,416 contigs were obtained, and the mean contig size was 138 bp with lengths.
The mean contig size was 831 bp, with lengths ranging from 201 bp to 17,900 bp.
The mean contig size was 259 bp with lengths ranging from 100 to 9,135 bp, including 697 contigs larger than 2,000 bp.
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The N50 and mean contig sizes also increased, approaching that predicted for a complete transcriptome.
Even though the Multireads strategy produced the assembly with smaller median and mean contig sizes, larger contigs were reconstructed (Additional file 1: Table S3), thus explaining the better results obtained for the CDS reconstruction.
An overview of the sequencing and assembly is outlined in Table 1.The mean contigs size of the sample from MM treatment was 648 bp, with lengths ranging from 101 to 4750 bp, while that of the sample from AM treatment was 536 bp, with lengths ranging from 101 to 4234 bp (Table 1).
The average contig size was 23,788 bp for the RJ and 1,002 bp for the BA metagenomes.
The largest contig was 26 kb while the average contig size was 2.2 kb.
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