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The mean contig length increased to 454 bases.
The mean contig length and N90 contig length are also both shorter by the 454 data.
The de novo assembly had high contiguity, with a mean contig length of 1,102 bases.
Mean contig length was 621 bp for C. piger and 732 bp for C. riparius.
Mean contig length was roughly 1 kb for all three species (Table 3).
The mean contig length was 871 bases and the total length of assembled contigs was 83,601,386 bases.
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Normalizations not only showed the largest average and mean contig lengths but also ran considerably faster than the Multireads counterpart.
Based on assemblies of 0.4 to 4.1 million reads (depending on species), the average number of contigs (or isotigs) was 54,721, 56,955, and 20,598 using the MiraEST, NGen, and Newbler assemblers, with mean contig lengths of 787, 797, and 1198 nt.
As expected from the size-selection procedure used in the construction of the libraries, N50 was 321 bp and the mean contigs length was 312 bp.
The mean read length, contig length and GC % were 75.00, 519 bp and 44.74% for control and 76.15, 493 bp and 44.70 for wounding library.
A comparison of basic assembly statistics (Table 2) showed that the Trinity assembler outperformed all other platform/software combinations in terms of number of contigs, number of assembled nucleotides, mean and maximum contig length, and N50.
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