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Its closest sequenced relative is Yarrowia lipolytica, although mean conservation of orthologs is low.
CR is therefore a measure of how the mean conservation of a kmer differs from the background conservation within the sequences being analyzed.
This phenomenon is clear in a number of E. coli motifs, particularly GntR, which has a mean conservation of 0.74 bits/col; the synthetic data results suggest relatively low values of sSn and sPPV for this motif.
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However, the key question is whether the conservation of expression patterns of network constituents necessarily means conservation of functional network interactions.
Values were averaged over the 50 terminal intronic nucleotides to yield a mean conservation level of that region.
The mean conservation scores of the seed pairing regions were then calculated.
The degree of conservation was ranked high only if it fell within one standard deviation range from the mean conservation degree of mtDNA disease-causing mutations [ 31].
(1) Masking _ C i = C i − 1 2 n + 1 ∑ i − n i + n C j (2) Filtering _ C i = Masking _ C i, Masking _ C i > 0 0, Masking _ C i ≤ 0 Masking_ C i is the mean conservation score of residue i, C i is the standard conservation score in PSSM, 2n+1 is the masking window size.
Conservation Index scores X ≥ 7 were considered as having the highest functional potential, being within 1SD from the mean Conservation Index scores of mtDNA disease-causing mutation (8.4 ± 1.8, see also results).
Conservation index scores X ≥ 7 were considered as having the highest functionality, being within 1 SD from the mean conservation index scores of mtDNA disease-causing mutation (8.4 ± 1.8, according to Levin et al. 2013).
Whereas the conservation of the synthetic motifs used here is independent of position, Eisen (2005) notes that real TFBS motifs with low mean conservation often have clusters of well-conserved positions; we believe that differences in the distribution of high and low conservation across true motifs in comparison with synthetic motifs explains the improvement in performance on real data.
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