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At 72 h, the mean colony counts after inoculation in experimental femurs were significantly lower than those of the control group; the reduction percentage in the total number of bacteria adhered to the femur being 76%% (p = 0.041).
On comparing paired samples (control and irradiated for implants and femurs) there were no significant differences in mean colony counts after inoculation between the control and experimental femurs at 24 and 48 h.
To assess the efficacy of UV radiation, the mean colony counts after inoculation in the experimental subjects and the control group were compared at different times of sacrifice and at different inoculum doses.
At 72 h, the mean colony counts after inoculation in experimental femurs were significantly lower than those of the control group, with a reduction percentage of 76 % (p = 0.041).
A similar difference between control and experimental implants was observed at 24 h using inoculum doses <104 CFU; the mean colony counts after inoculation in the UV-irradiated implant were significantly lower than those of the control implant with the reduction percentage being 70.48 % (p = 0.017) (Fig. 5).
Surviving fraction was calculated as (mean colony counts)/ cells inoculated)× plating efficiency (PE)), where PE was defined as (mean colony counts)/ cells inoculated for non-irradiated controls).
Similar(47)
Each experiment was performed in triplicate and the mean colony count for each experiment was converted to values relative to the mean colony count at 0 h to normalize the data and correct the variation in starting inocula concentrations.
The mean colony count was 44 × 10 CFU/mL (range: 50 to > 10).
The mean colony count, after discharge, on the 66 bed decks was 6.7 cmU/30 cmedianian 1 cmU/30 cm ; SD 20.2).
The mean colony count of the two plates was used to derive the log10 CFU/ml per swab.
The concordance between sTA and diagnostic test was function of mean colony count and of time interval between the sTA and VAP onset.
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