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d Comparison of total cell number and mean cell length in the outer glume surface of spikelet hull.
b Comparison of total cell number and mean cell length in the cross-section of outer glume cell layers of spikelet hull.
Dotted line indicates position of cross-section (b) Magnified view of spikelet hull cross-section boxed in a. (c) Comparison of total cell number and mean cell length in the outer glume cell layers of spikelet hull of Ilpumbyeo, group D, and group J. (n = 5 to 8 spikelets).
from the mean cell length.
This leads to an increased normalized s.d. of the mean cell length with a value of 24% (N = 19).
We set the mean cell length to one and plotted the respective normalized standard deviations (s.d).. Low values of the normalized s.d.
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(C ) Mean epidermal cell length in the root meristem of DMSO or WM (2 µM; 20 hr) treated seedlings.
Consistent with this is that in cortical tissue adjacent to young lateral root primordia, mean cortical cell lengths of 75 ± 10.1 and 56.86 ± 10.3 μm for WT and Spcdc25, respectively were not significantly different (n = 25, see Additional file 2).
We measured that the partition complex covered a distance of 1.0 ± 0.2 µm along the cell length (mean ± standard deviation, SD, n = 141 cells) within 4.7 ± 1.2 min during this fast ParA-dependent phase, with an average velocity (run length/run period) of 220 ± 50 nm/min.
These microscopic analyses revealed that both Δ ZtCBR1 strains exhibited a significant overall decrease in individual cell length (mean length, WT = 11.14 μm, Δ ZtCBR1- 1 = 7.17 μm, Δ ZtCBR1- 2 = 7.97 μm, p < 0.05) and increase in the proportion of single-celled spores (binomial test Δ ZtCBR1- 1 vs WT, p = 0.003, Δ ZtCBR1- 2 vs WT p < 0.001) (Fig. 4C and D).
Cellular densities were calculated using the ratio between the mean cell number and the mean length of the both entire sections (Wright Cell Imaging Facility, ImageJ software, National Institutes of Health, Bethesda, MD, USA).
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