"Many of the extracted features have obvious or intuitive interpretations, such as mean cell area, eccentricity, intensity.
The mean cell area and cell fluorescence intensity in five areas and 2(C)) were evaluated as described in previous studies [ 13, 14].
Modulation of fluid flows was observed to effect an immediate, reversible change in mean cell-wide fluorescent intensity.
When analyzing the level of EGFP per cell, the mean EGFP intensity levels HEK293 cells were not significantly different in six out of the eight novel vector constructs.
In contrast, significantly lower expression of SAA was detected in Epstein Barr transformed B cells (LCL) and cervical cancer cell lines (CVX) used as controls (i.e., 65 71% positive cells; mean fluorescence intensity range from 11 to 20 and 69 84% positive cells; mean fluorescence intensity range from 9 to 15, respectively) by flow cytometry (P=0.03 for both USPC vs LCL and USPC vs CVX, Table 1).
Minimal expression of cell surface OAcSGP (5 10% of OAcSGP+ cells, mean fluorescence intensity, MFI, being ~10 50 au) on PBMCN was evidenced using FITC-Achatinin-H (0.04 μl/10 μl).
In fact, there was a two-fold enhanced anti-CD22 binding to CD27negative B-cells (mean fluorescence intensity (MFI) 324.7 ± 74.4) compared to CD27positive B-cells (MFI 172.9 ± 40.9) (P = 0.0002).
Average ± SEM of normalized mean intensity per cell (n=11 cells, Bem1; n=14 cells, Cdc24).
Pgp was expressed at low level in A2780 and A2780/DDP cells (mean fluorescence intensity being 8.5 and 7.9, respectively), whereas an increased expression was found in INT.ACP/PTX cells (mean fluorescence intensity, 25).
