Hb concentration was calculated from the Hct as follows: Hb (g/dl) = Hct×0.34 (Mean cell Hb concentrations were assumed to be comparable in all rats).
Also the accompanying indices of mean corpuscular volume (MCV), mean cell Hb (MCH), and red cell distribution width (RDW) were analyzed.
We also used these tests to compare Hb and related parameters such as mean cell volume [MCV], mean cell Hb [MCH], haematocrit [HCT] and CD4 cell count at follow-up time points.
Mean cell Hb (amount of Hb/red blood cell) and platelet count (number of platelets in blood volume) will also be calculated, and (3) Arterial O2 Saturation will be assessed at rest and continuously during exercise using pulse oximetry (Biox 3700, Ohmeda Medical, Boulder, CO), which provides the most accurate noninvasive assessment of blood arterial O2 saturation levels.
There were significant differences between placebo and MCTD groups in creatinine (83·62 (sd 17·08) μmol/l v. 79·67 (sd 15·02) μmol/l, P=0·025) and mean cell Hb concentrations (34·8, 33·5 35·0 v. 34·0, 33·0 34·7 g/dl, P=0·030), both of which were higher in the placebo diet group.
The analyzer aspirates the blood sample, dilutes and counts leukocytes, erythrocytes and thrombocytes, measures Mean Cell Volume (MCV) and Haemoglobin (Hb), and calculates Haematocrit, Mean Cell Haemoglobin (MCH), and Mean Cell Haemoglobin Concentration (MCHC).
White blood cells (WBC), lymphocyte and neutrophil ratio, red blood cells (RBC), hematocrit (Hct), hemoglobin (Hb), mean cell volume (MCV), mean cell hemoglobin (MCH), mean cell hemoglobin concentration (MCHC), and platelet count (PLT) were measured on Hematology Analyzer (Abacus Junior Vet 5, Austria).
Hb: hemoglobin; IL-6: interleukin 6; MCH: mean cell hemoglobin; MCV: mean corpuscular volume; RDW: red cell distribution width; SIRS: systemic inflammatory response syndrome; TOF-MS: time-of-flight mass spectrometry.
Parameters evaluated include packed cell volume (PCV), red blood cell (RBC) count, hemoglobin (Hb), platelet count, total and differential white blood cell (WBC) count, mean cell hemoglobin concentration (MCHC), mean red cell volume (MCV), and mean cell hemoglobin (MCH).
The CBC included WBC (total and differential count), RBC count, RBC size (mean cell volume [MCV]), Hb content (mean corpuscular hemoglboin [MCH] and mean corpuscular hemoglobin concentration [MCHC]), total Hb concentration (HGB), hematocrit (HCT), and platelet count (PCT).
The RDW value, hemoglobin (HB), mean cell volume (MCV), platelet, and white blood cell (WBC) were determined using an automated blood cell counter with an automated hematology analyzer XE-2100 (Sysmex Corporation, Kobe, Japan).
