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The accuracy of each assay was determined by analysing QC samples at three concentration levels in replicates (N = 6, per concentration level) and comparing the mean calculated values with the respective nominal concentration values.
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The mean calculated rotation values for five observers are given in Table 2.
The mean calculated molecular property values for compounds were compared using the t-test and ANOVA with JMP v. 8.0.1 (SAS Institute, Cary, NC).
Then it calculates the standard deviation of samples from the difference of expressions and fits logarithm of the linear function SD = a1 + a2 Y mean (1) to the logarithm of calculated values; here Y mean is the sample mean and a1 and a2 are the intercept and coefficient proportionality, respectively.
* SAM p-value less than 0,001 for every sample ** amplitude of deviation from the mean calculated from the RMA values The megakaryoblastic specific genes assigned with a functional annotation (GO terms) were divided into different functional classes.
In brief, the top and bottom 2% of the signal values were removed and the mean calculated for the 96% of the values remaining.
Measurements were taken twice and if agreement between repeats exceeded 0.4 cm, a third measurement was taken and the mean calculated using the closest two values.
The mean %error in calculated value from the expected 2.5 ng/mL for 2 and 18 h combined was lower for plasma samples at 7.7 ± 6.8 % compared with serum at 17 ± 14 % suggesting that factors present in serum may reduce accuracy and produce more variability in LTx activity.
We decided post-hoc to perform a sensitivity analysis of studies without calculated values (median instead of mean; SD calculated from other studies; adjusting for baseline values).
More precisely, the calculated values of the mean invasion probability for the larger grid size were within the standard deviation of the mean obtained by the smaller grid size, and vice versa.
For example, if a participant has a missing value for question 17, the imputed value is the mean calculated from the completed question 17 for the entire cohort.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com