The mean cortical bone thickness (CBT) and the mean trabecular bone thickness (TBT) were measured in 5 fields/slide from 5 slides for each rat.
For determining the diameter of the vessels, the sphere algorithm (implemented in the CtAn software) and routinely used to measure the mean bone trabecular thickness was used [22].
The mean buccal bone thickness measured by observers 1 and 2 was 2.42 mm (sd: 0.50) and 2.41 mm (sd: 0.47), respectively.
The mean buccal bone thickness of the first measurement and the second measurement of observer 1 was 2.42 mm (sd: 0.50) and 2.53 mm (sd: 0.49), respectively, with an intraobserver intraclass correlation coefficient of 0.93 (95% CI 0.88 to 0.96).
The mean buccal bone thickness of the first measurement and the second measurement of observer two was 2.41 mm (sd: 0.47) and 2.52 mm (sd: 0.47), respectively, with an intraobserver intraclass correlation coefficient of 0.96 (95% CI 0.93 to 0.97).
The measurements were expressed as a percentage of the mean ratio of bone thickness to the total tissue thickness (BT/TT) of the nine regional measurements in the five adjacent sections.
Consistent with this, the mean ± SD measurement of subchondral bone thickness in this study, in each of the non-OA compartments and the OA lateral compartments, was 1.2 ± 0.23 mm.
The local bone mineral density and the trabecular microstructural parameters, including the mean bone volume to total volume (BV/TV), trabecular thickness, trabecular separation, and structure model index (SMI), were measured using bone analysis software.
For the cortical bone surrounding the trabecular volume of interest, the average cortical bone area (Ct.Ar), cortical bone thickness (Ct.Th), and bone marrow area (Ma.Ar) were computed.
Mean bone ingrowth distance was 2.79 (SD 0.76) mm and the mean thickness of the whole graft layer was 5.56 (SD 0.85) mm.
