The mean binding ratios (n=3), as semiquantitatively determined with western blotting, are shown in Figure 2e, with IOD standardized and the ratio at 0 μM of ATM defined as 100%.
An average binding ratio was calculated ± one standard deviation.
In each healthy control subject, [123I]FP-CIT binding was higher in the hypothalamus than in the cerebellum, and the mean hypothalamic binding ratio of SERT was 0.29 ± 0.23.
In each healthy subject, [123I]FP-CIT binding was higher in the hypothalamus than in the cerebellum, and the mean hypothalamic binding ratio of SERT was 0.29 ± 0.23.
Data was initially normalized between different arrays (method = 'vsn') and subsequently to the mean log2 binding ratio in the euchromatic part of 2R.
The mean hypothalamic SERT binding ratio of the present study appeared to be lower than previously reported in the diencephalon [23], possibly related to a more precise delineation of the target area in the present study and to age differences.
Using these empirically determined relationships, we can use m, the mean binding site number, V/m ratio, and the clade age to estimate the model parameters.
In order to explore the evolution of binding site density, we computed the mean binding site density and the ratio of binding site variance to mean (V/m ratio) for various clades of yeast species.
Because the λ/μ ratio strongly influences the expected mean binding site number among taxa, this ratio is much easier to estimate than the individual parameters, λ and μ.
For example, we found that PRE and ERE densities in HoxA clusters are heterogeneous among gnathostomes, comparison of V/m ratios, and mean binding site densities indicate that the heterogeneity is between mammals and the other gnathostomes.
