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Final results were blank corrected using the mean batch blank value.
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To evaluate contamination from laboratory, sampling matrices, and sample handling, we analyzed field blanks (n = 4 neutrals; n = 3 phenols), batch blanks (n = 5), and matrix blanks (n = 5 phenols, 6 neutrals).
Before: comparison was performed prior to mean batch-centering.
After: comparison was performed following mean batch-centering.
An independent mind doesn't mean a blank mind.
The mean background (blank) MFI was then subtracted, generating netMFI.
Cell viability for each sample was calculated using (3) Cell survival % = Mean of each group − mean of blank mean of negative control − mean of blank × 100.
Mean was calculated from triplicate wells and subtracted from mean of blank wells resulting in ΔFluorescence.
Cell death induction was calculated as 100 × (1− (OD exp. mean value (−substrate blank)/OD control mean value (− substrate blank)).
Mean blank values were subtracted from sample values for each batch.
Six procedural blanks were analyzed in the same batches as the samples and concentrations were blank-corrected by subtracting the mean blank values (in pg) from the raw analyte values.
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