Sentence examples for mean base quality from inspiring English sources

As seen in the figure, mean base quality decreases in an almost linear fashion as more bases are read.

First, the methylation information for each cytosine including the coordinates, sequence context (CG, CHG, CHH), number of methylcytosines, read coverage and mean base quality (PHRED) score.

To ensure that low quality reads were excluded from the analysis, the raw sequence reads were filtered to remove any entries with a mean base quality score < 20.

An alternate approach is to use the mean base quality for a read; however, the mean does not capture the base position-dependent nature of base quality scores.

21,095,918 readseq reads (100 base-pairs long) were trimmed to 85 bases where the mean base quality score was above 24 (Illumina 1.5+, phred + 64) using the FASTX-Tool kit (Ver.0.0.13).

For example, across the 20 T. alpinus libraries, the overall mean base quality score was 33.7 ± 0.1, while 84.7 ± 0.3% of the bases in the raw sequence reads had quality scores greater than 30.

Therefore, in the rest of this article, the term "packet" means base quality layer packet or MGS packet.

The gap distribution for unigenes is shown in Figure 2. * Total Nucleotides = Total Reads1 x Read1 size + Total Reads2 x Read2 size, Q20 means base quality more than 20, N represents unknown nucleotides.

Read quality was checked with FASTQC and filtering was performed on the reads with PrinSeq-Lite to ensure a mean base-pair quality of score greater than 20.

FastQC [ 14] overview of raw sequences showed that mean per-base quality remains above 24 for the first 350 bp and, thereafter, drops rapidly towards the end of the reads (data not shown).

For each HQD type, the mean and median base quality score were calculated (see Methods).