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Total mean base coverage is (total sequenced bases/total targeted bases).
Although coverage at 20 × (C20) varied, it correlated to mean base coverage as expected.
Also, C1 increased to 99.4-99.7% when mean base coverage was greater than 1000×.
The SNP detection rate correlated with the mean base coverage, and was unaffected by the pooling strategy.
For samples with mean base coverage of at least 200 reads per base, C20 was at least 90%.
When total mean base coverage was similar, FS had more calls (215) than SHS-PE low (179).
Similar(47)
The mean length of the annotated transcripts was 1,297 bp and the average base coverage was 54X (median 11X).
The average base coverage within target exons for individual T. alpinus libraries ranged from 7.1 to 13.7X, with a mean of 10.1X (Additional file 5).
5′/3′ Coverage: the mean per-base coverage for end regions of RNA transcripts.
We excluded ORFs that lacked a mean per-base coverage ≥10-fold in both orthologs, but retained 3091 NC genes as well as 74 ORs, four IRs, one GR, 10 OBPs and five CSPs (Table 2).
We used the pooled read sets representing 40 males per species to infer sites fixed for differences between all detected orthologous NC and chemosensory genes with ≥10-fold mean per-base coverage in Euglossa dilemma and E. viridissima.
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