Sentence examples for mean background signal from inspiring English sources
Sample signals were corrected using this mean background signal.
For detected spots, their signal intensities were determined after subtracting with the mean background signal.
Features with signal intensities less than 2.5 fold of the mean background signal were removed, and ratios were normalized using housekeeping gene normalization [ 16] and were averaged and log transformed.
EGFP intensities were measured by drawing a rectangular area of the same dimensions in the bleached and unbleached area followed by subtraction of the mean background signal outside of the cell.
For linkage analysis in the population of segregants, transcripts with a low signal-to-noise ratio were filtered out: we required the mean red (Cy5) signal to be three times greater than the mean background signal (Cy5).
For analysis of the transfected cells using flow cytometry, single cells were isolated (Supporting Information Figure 1), followed by calculating the mean fluorescence intensity (MFI) of the gated cells and subtracting the mean background signal.
Array images that did not pass minimal quality control were discarded (median signal-to-background >3; median signal-to noise >3; mean of median background signal <200).
Array images that did not pass minimal quality thresholds were not used (median signal-to-background >3; median signal-to-noise >5; mean of median background signal <200).
Array images were only used if the following quality thresholds were passed: median signal-to-background >8; median signal-to-noise >10 and mean of median background signal <250.
For each spot, mean local background signal was subtracted from the mean spot signal, negative signals were excluded, and the resulting net spot signal data were log transformed.
The ratio of mean spot signal to mean background variation (signal-to-noise ratio) was clearly lower for Alexa 488 then for the other dyes (namely, 1.25, 1.65, 2.88 and 1.88 for Alexa 488, Alexa 594, Cy3 and Cy5, respectively).
