Sentence examples for mean background from from inspiring English sources
Both, the series of DP retinal images and the background images were averaged, and a final image was obtained for each eye as the subtraction of the mean background from the mean DP image.
The mean background intensity from control and anti-E-selectin-targeted animals was subtracted.
The mean background determined from the intensity histogram of recorded image stacks was subtracted [36] and the resulting image stacks were used to automatically define peak regions of interest of synaptic bouton size [37], where AP-evoked fluorescence decrease (FM 1 43 and anti-synaptotagmin-1-cypHer) or increase (synaptopHluorin) occurred in difference images.
The final mean fluorescence intensity was calculated by subtracting the mean background value from the mean signal value.
In all these instances, the mean background intensity from an area outside of the cells was subtracted for each pixel.
Intensity thresholds were set to include all pixels equal to and greater than 1.45× the mean background fluorescence from the cell (to control for background fluorescence and minor variation in fluorescent expression level).
The results were calculated by subtracting the mean background values from the values obtained under each test condition and were expressed as the percentages of the control (untreated cells) values.
The results were calculated by subtracting the mean background value from the values obtained under each test condition: values were normalised to the number of cells in each well and were expressed as the percentages of the control (untreated cells) values.
From these scans, we obtained a relative transmissionaTrel(red dashed line in Figure 1) by normalizing the measured intensity Im with the mean background intensity Ib from regions without particles: Trel=Im/Ib.
After the registration and deformation of the images, each voxel in a VOI of the 18F-FAZA images was normalized to the mean background value (obtained from corresponding VOIs posterior of the sternum) in order to calculate a T/Bg.
Specific green fluorescence emitted by FITC-microspheres was determined by subtracting the mean background fluorescence calculated from three different areas of the image where no cells were present, from the overall mean green fluorescence of the entire image.
