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For each background frequency, the mean displacement for 'gap' and 'no-gap' startle data was calculated.
A luminance matched grey target, high contrast stripe target (spatial frequency of 1.74 cycles/deg) and a 'background sample' camouflage target were all luminance matched to the mean background level.
Both CAQ and PAQ were corrected for mean background counts.
The fold-difference in TFBS frequency is computed by dividing the observed TFBS frequency by the background frequency.
General background signal in the GFP channel was corrected by automatic mean background subtraction.
Mean, background subtracted fluorescence was averaged for triplicate infections.
The mean background (blank) MFI was then subtracted, generating netMFI.
We adjusted mean signal intensity for local background by subtracting the mean background intensity.
The background frequency was determined on 100,000 pseudo-SBE sequences.
For a large sized background, each column of the M ref approximates the nucleotides background frequency.
a [Sample frequency]/[Background frequency] bSampled genes exclude hypothetical proteins with no assigned function.
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