Sentence examples for mean background fluorescence from inspiring English sources

Intensity thresholds were set to include all pixels equal to and greater than 1.45× the mean background fluorescence from the cell (to control for background fluorescence and minor variation in fluorescent expression level).

The threshold for significance was defined as any pixel with a value greater than or less than 2.5 standard deviations of the mean background fluorescence.

Specific green fluorescence emitted by FITC-microspheres was determined by subtracting the mean background fluorescence calculated from three different areas of the image where no cells were present, from the overall mean green fluorescence of the entire image.

To avoid a biased user-defined threshold, the fluorescence signals were segmented applying a threshold value equal to the mean background fluorescence intensity ±2 S.E.M. Cell bodies were excluded for this analysis.

The threshold cycle (Ct) was defined as the cycle at which the fluorescence of the samples crossed two standard deviations above the mean background fluorescence of the negative (uninfected human blood DNA).

The mean background fluorescence of resazurin was subtracted from all experimental data.

Mean, background subtracted fluorescence was averaged for triplicate infections.

This was subtracted from the fluorescence for the cell surface ROI, yielding a mean background-corrected fluorescence.

Briefly, the signal was outlined and the mean intensity and background fluorescence were measured.

Frustrated by our inability to improve the intrinsic kinetics of the Staudinger ligation, we turned to alternate means of reducing background fluorescence in cell imaging experiments.

Both background fluorescence and mean fluorescent intensities of ROIs were calculated in each image.