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These densities were then normalized by dividing the area of the box and corrected by subtracting the mean background density per unit area.
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To ensure the signals obtained were within the linear range, the mean background-corrected optical density (OD) of each signal was interpolated for an OD calibration curve created using a calibrated OD step tablet (Kodak).
A curve-derived theoretical L.O.D. could be considered viable up to 6.63×104 mg−/mg, as this was the corresponding tissue-ratio at which the diglycosylated band's density curve intersects the mean background of the blot.
A neuron was considered positive when its mean silver grain density was greater than image background density (averaged from 3 separate ROIs over slide background) plus two times the standard deviation of this density.
The background density was set to 0 g/cm3.
The mean sensitivity was 0.26 ± 0.09 for film and 0.20 ± 0.07 for digitized images (P =.000), with a pronounced decline in the latter in regions of high background density.
We consider these minimum values as background density and velocity.
The actual maximum value of the induced density is around 50 in units of background density.
In these cases, the peak density is very close to the E region background density.
The color map shows the change in density in units of the background density n0.
Both CAQ and PAQ were corrected for mean background counts.
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