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In an analysis of all acquired images, the mean background contribution is increased by 30% from 70too 100 cps.
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Particularly, Eq. (33) corrects the linearized signal (Eq. (32)) for the probe-specific background contribution using the mean background of the chip and the probe-specific sensitivity for non-specific hybridization, L p P, N (x, μ c P ) = 10 δ A p P, N + μ c P + x (Eq. (23)).
The 4% signal-to-background ratio from the average line profile yields an upper limit to the nonresonant background contribution.
The background contribution was refined in the final fit.
Assuming a non-fluctuating background contribution, this can be simply subtracted from the original data.
Both CAQ and PAQ were corrected for mean background counts.
General background signal in the GFP channel was corrected by automatic mean background subtraction.
Mean, background subtracted fluorescence was averaged for triplicate infections.
The mean background (blank) MFI was then subtracted, generating netMFI.
We adjusted mean signal intensity for local background by subtracting the mean background intensity.
This large background contribution might have been inflated by the implicit inclusion of the model error.
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