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Non-neoplastic brain samples had mean average methylation of 3.2±2.89 s.d.
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MSP data showed insignificant (p = 0.08) difference in the average mean methylation level between the groups, while the pyrosequencing analysis revealed the change in mean methylation level from 1.4 ± 0.57 % to 11.5 ± 2.25 % between the control and glioma patients, which was significant (p < 0.05) (Fig. 2g).
To determine the difference between average mean methylation levels and to analyze the independence of CpG methylation between the control and glioma patient groups, a two tailed Mann–Whitney U test was performed.
The number of sample is S A for group A, and S B for group B. (Equation 2) AVG R,G means the average methylation level of group G at the R region.
Mean averaged reciprocal rank.
Lie with mean averages.
Fractional methylation of a promoter region was calculated by averaging methylation of all CpG sites in that region.
The other is called differential averaged methylation rate (dmr).
The average CGI methylation was obtained by averaging methylation percentage of all the CpGs in a defined CGI.
K-means classification of average methylation values computed over that set identified 6, 10, and 7 cell lines as CIMP+, CIMPi, and CIMP−, respectively.
mean percentage of methylation over the respective methylation sites as continuous variable and (2).
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