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The mean average intensity of ≥100 cells was quantified.
As in the case of the peak and mean (average intensity) ratings, a significant main effect of itch stimulus was detected in the time course data (p<0.0001), and this effect did not vary with disease status (drug×disease status interaction: p = 0.91).
Net signal was determined by subtraction of this local background from the mean average intensity for each spot and the nospecific influence of cross-hybridization from negative control.
Preliminary analysis of Illumina data was performed using Illumina BeadStudio software which returns the trimmed mean average intensity for each single gene probe type (non-normalized).
Quantification of apoptosis was performed by measuring caspase-3 activation and nuclear fragmentation, wheras quantification of cell cycle arrest was obtained by nuclear DNA content (mean average intensity of DAPI) and phospho-histone H3 (total intensity).
Mean average intensity of benzene exposure for AML cases and controls was 0.18 p.p.m. and 0.2 p.p.m., respectively, and for CLL cases and controls was 0.19 p.p.m. and 0.21 p.p.m., respectively.
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Quantitation of the EB red fluorescence emission, measured as the intensity per area unit (mean gray value: averaged intensity per pixel), was done in the whole striatum and parietal cortex of vehicle- and GA-injected animals by using the Image J software (NIH, USA).
For each antigen LMC and Ts65Dn normalized mean intensity values were divided by the average mean intensity for all LMC samples to obtain the percentage of LMC.
This fish species is frequently infected with A. pegreffii in the Mediterranean Sea, with an average prevalence of infection in the Tyrrhenian Sea P 1.00% and an average mean intensity, Im 1.00 (our unpublished data).
Flow cytometry was then performed and results represent the average mean intensity (N=3).
The average mean intensity of mf reaching the hemocoel was 15.5 ± 19.2 per mosquito.
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