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The mean allelic transcript ratio for the whole assay was then calculated, and normalised to the mean allelic transcript ratio for the genomic controls.
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Across loci, mean allelic richness was similar across regions, while private allelic richness was highest in the core locality (corrected for sample size).
Â20, mean allelic richness normalized to the smallest sample size; SD, standard deviation of Â20; UAN, unique allelic number.
For the seven populations, mean allelic richness varied between 1.53 and 3.29.
The lower accuracies under scenario B are more evident for Bovine3K which has much higher mean allelic error rate (5.52%).
These 10 polymorphic markers (Table 1) had mean allelic richness of 10.3 alleles per locus (Table 2).
4 Rarefaction estimates of mean allelic richness and mean number of private alleles per locus.
For each population and each population pair we used the mean number of alleles per locus, the mean expected heterozygosity and the mean allelic size variance.
At nuclear loci, expected heterozygosity was quite similar across populations whereas mean allelic richness was more variable (Table 1).
For a given cDNA or gDNA assay the allelic transcript ratio was calculated on each of the 9 technical replicates from the relative quantity of the two allele-specific transcripts.
Allelic diversity within each population was the mean allelic diversities among the 114 loci.
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