For each cell, new anovas were used to measure category, location and object selectivity based on mean activity in two analysis windows, early (50 250 ms from choice stimulus onset) and late (300 500 ms).
We repeated the experiments at least three times and quantified 'the mean activity of Rac activation' by densitometric analysis.
Fig. 11 Luciferase activity analysis (mean ± SD, n = 4) after transfection of HeLa with pGL-3/PEI 25 kDa, pGL-3/Pluronic-PEI, pGL-3/Pluronic-PEI-DR5, pGL-3/Pluronic-PEI-TAT, or pGL-3/Pluronic-PEI-DR5-TAT at different N/P ratios.
*P < 0.05, **P < 0.01 Fig. 12 Luciferase activity analysis (mean ± SD, n = 4) after transfection of HepG2, with pGL-3/PEI 25 kDa, pGL-3/Pluronic-PEI, pGL-3/Pluronic-PEI-DR5, pGL-3/Pluronic-PEI-TAT, or pGL-3/Pluronic-PEI-DR5-TAT at different N/P ratios.
*P < 0.05, **P < 0.01 Fig. 13 Luciferase activity analysis (mean ± SD, n = 4) after transfection of NIH 3T3 cells with pGL-3/PEI 25 kDa, pGL-3/Pluronic-PEI, pGL-3/Pluronic-PEI-DR5, pGL-3/Pluronic-PEI-TAT, or pGL-3/Pluronic-PEI-DR5-TAT at different N/P ratios.
For 42 cells with early discrimination (50 250 ms from stimulus onset; Fig. 7 E, G ), we compared mean activity in this early analysis period for target corrects, nontarget corrects, and nontarget errors.
In order to perform this analysis the mean activity coefficients of the salt in the presence of DMSO were experimentally determined and the electrical double layer parameters calculated by using Fawcett's function.
Non-normalized spiking activity was calculated as the mean activity (spikes/s) averaged over the analysis period (200 3000 ms after stimulus onset) given a specific stimulus.
Although analysis of spatial mean activity can predict score improvements to some extent, it provides merely summary statistics of the activity in an ROI, ignoring subtle differences in activity patterns.
