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Using the mean absorbance values for each sample, corresponding concentrations were determined after plotting the standard calibration curves.
Mean absorbance values of six readings of two replicates (three each) were taken into the consideration for calculation of the concentration of arsenic.
The mean absorbance values obtained for the standards and the samples were divided by the absorbance value of the zero standards and multiplied by 100.
The activity in experimental samples was ascertained using the standard curve of mean absorbance values (OD) versus the dilution of recombinant IFN-γ in the supernatant.
For both assays, the mean absorbance values of at least three independent experiments were used for statistical analysis.
Samples were considered positive if their absorbance values were greater than two times the mean absorbance values of negative controls.
Similar(33)
The mean absorbance value of cells non-treated with supernatants was taken as 100% cellular viability.
The cut-off value was calculated as the mean absorbance value of the negative controls plus three standard deviations, as obtained with a single NASBA-ELISA test.
The cell growth inhibition (CGI) ratio was calculated from the absorbance values through the following formula: {text{CGI }} = left( {{text{C}} - {text{T}}/{text{C}}} right) times 100 where C is mean absorbance value of untreated (control) cells and T is mean absorbance value of treated cells [40, 41].
As the mean absorbance value and standard deviation were 0.059 ± 0.012, a sample was considered positive when the absorbance of the three measurements was greater than 0.095 (the cut-off value).
The cut-off value was four times the mean absorbance value of a negative control serum at a 1∶100 dilution.
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