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Mean absorbance obtained for control condition was considered 100% of cell viability.
Figure 2 shows no relevant values (data expressed as the mean absorbance obtained after densitometry quantification) of the MMP-2 in both sides of masseter muscle from the experimental and control groups.
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The top panel of Fig. 5 shows mean absorbance for the condition without an air-leak for the 19 subjects (solid line) from whom reliable data were obtained.
The viability of cells was calculated as: [ mean absorbance of erlotinib-treated wells − mean absorbance of cell-free wells)/ mean absorbance of vehicle wells − mean absorbance of cell-free wells)] × 100.
The mean absorbance values obtained for the standards and the samples were divided by the absorbance value of the zero standards and multiplied by 100.
The IC50 values were graphically obtained by plotting the absorbance obtained against the corresponding different concentrations of the test compound used, and are reported as the mean ± 1 standard error (SE).
The absorbance obtained from treated cells were expressed as percentages of absorbance obtained from untreated cells and are reported as mean ± SEM (n =3).
Plots of sensitivity (absorbance/concentration) versus absorbance obtained with a suitable testing solution yield both the linear range and the effective path length of the detector.
Background correction was achieved by subtracting the changes in absorbance obtained with BSA.
The absorbance obtained at each time point was normalized to the absorbance at 0 hours.
IgE titers were expressed as the absorbance obtained by subtracting the absorbance of the serum from control group.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com